rabbit polyclonal anti mef2d  (Bethyl)

Journal: iScience

Article Title: Dissection of the MEF2D-IRF8 transcriptional circuit dependency in acute myeloid leukemia

doi: 10.1016/j.isci.2022.105139

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Article Snippet: Rabbit polyclonal anti-MEF2D , Bethyl Laboratories , Product #: A303-522A.

Techniques: Recombinant, Reverse Transcription, Magnetic Beads, Protease Inhibitor, Cell Viability Assay, Cloning, Purification, Isolation, Sequencing, Software

Journal: OncoTargets and Therapy

Article Title: miR-30a suppresses osteosarcoma proliferation and metastasis by downregulating MEF2D expression

doi: 10.2147/ott.s102430

Figure Lengend Snippet: Figure 4 MEF2D is required for miR-30a-directed osteosarcoma cell proliferation, migration, and invasion. Notes: (A) MEF2D expression was increased by transfection with MEF2D plasmids in miR-30a mimics osteosarcoma cell. (B) The MTT assay was used to detect MG-63 cells cotransfection with miR-30a mimic and pcDNA-MEF2D plasmid. (C) The wound healing assay was performed to detect the effects on cell migration of MG-63 cells treated as described. (D) The transwell invasion assay was used to detect the effects on cell invasion of MG-63 cells treated as described. Data are presented as mean ± SD from three independent experiments. *P,0.01 vs miR-30a group. **P,0.01 vs miR-30a + MEF2D group. Abbreviations: h, hours; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; OD, optical density.

Article Snippet: Then, the protein samples were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (10%) and detected by Western blot using polyclonal (rabbit) anti-MEF2D antibody (Santa Cruz Biotechnology Inc., Dallas, TX, USA).

Techniques: Migration, Expressing, Transfection, MTT Assay, Cotransfection, Plasmid Preparation, Wound Healing Assay, Transwell Invasion Assay